Application of Thin-Layer Chromatography-Flame Ionization Detection (TLC-FID) to Total Lipid Quantitation in Mycolic-Acid Synthesizing Rhodococcus and Williamsia Species
نویسندگان
چکیده
منابع مشابه
Analysis of Heavy Oils by Thin-Layer Chromatography with Flame Ionization Detection†
Several rapid methods for compositional analysis of heavy oils by thin layer chromatography with flame ionization detection (FID-TLC) were investigated. Separating into four fractions, Saturates, Aromatics, Resins, and Asphalten, SARA analysis of heavy oils could be accurately performed by three stage development using n-hexane, toluene, and tetrahydrofuran. Detailed hydrocarbon type analysis o...
متن کاملDynamics of bitumen fractions by thin-layer chromatography/flame ionization detection
Thin-layer chromatography/flame ionization detection (TLC/FID) allows for the fractionation of bitumen into four fractions, namely, the saturates, the aromatics, the resins A and the resins B (SARAB). The technique is rapid and economical, but it lack reproducibility. The effect of chromarod aging on reproducibility was investigated along with that of the time between bitumen dissolution and it...
متن کاملSeparation and quantitation of molecular species of plant phosphatidylcholine by high-performance liquid chromatography with flame ionization detection.
A method is described for the direct quantitation of phosphatidylcholine molecular species by reverse phase high-performance liquid chromatography employing flame ionization detection. The method is shown to be applicable to plan phosphatidylcholine. The molecular species are separated with a C18 column eluted in an isocratic mode. Detection by a commercially available flame ionization detector...
متن کاملApplication of thin-layer chromatography to the quantitation of plasma neutral lipids and free fatty acids.
THIN-LAYER CHROMATOGRAPHY (TLC) has been widely applied to the qualitative analysis of complex biologic lipid mixtures (1, 2), as well as to the quantitation of selected biochemical entities (3). Quantitation by TLC has been limited,for the most part,to the recovery of separated components from the chromatoplate, followed by an independent analysis using established analytic methods. Current te...
متن کاملif . Thin Layer Chromatography / Flame Ionization Analysis of Transesterified Vegetable Oils 1
A quantitative method was developed for analyzing mixtures containing fatty esters and tri(TG), di(DG) and monoglycerides (MG) obtained by the transesterification of vegetable oils. Analyses were performed by thin layer chromatography (TLC)/flame ionization detection (FlO) with an latroscan TH-10 instrument. Stearyl alcohol served as an internal standard. From plots of area and weight ratios of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2020
ISSN: 1422-0067
DOI: 10.3390/ijms21051670